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Related Experiment Video

Updated: Oct 2, 2025

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Oocyte Penetration Speed Optimization Based on Intracellular Strain.

Yaowei Liu1,2, Maosheng Cui2,3, Yidi Zhang1

  • 1Institute of Robotics and Automatic Information System, The Tianjin Key Laboratory of Intelligent Robotics, Nankai University, Tianjin 300071, China.

Micromachines
|February 25, 2022
PubMed
Summary
This summary is machine-generated.

Optimizing oocyte penetration speed significantly improves embryo development potential. Increasing speed to 50 μm/s reduces intracellular strain and enhances gene expression, boosting cleavage rates in porcine oocytes.

Keywords:
cell development potentialcell penetrationintracellular strainrobotic cell manipulation

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Area of Science:

  • Reproductive Biology
  • Biotechnology
  • Cellular Mechanics

Background:

  • Oocyte penetration is crucial for assisted reproductive technologies like ICSI.
  • Current robotic methods achieve high success rates but haven't improved post-penetration oocyte development.
  • Intracellular strain during penetration impacts oocyte viability.

Purpose of the Study:

  • To optimize oocyte penetration speed by analyzing intracellular strain.
  • To enhance the developmental potential of oocytes post-penetration.
  • To validate the optimized method at the molecular (gene expression) level.

Main Methods:

  • Analysis of intracellular strain at various penetration speeds.
  • Penetration experiments on porcine oocytes at different speeds (10 μm/s vs. 50 μm/s).
  • Assessment of oocyte cleavage rates and gene expression (totipotency, antiapoptotic) post-penetration.

Main Results:

  • Penetration at 50 μm/s reduced large intracellular strain by 80% and maximum/average strain by 25-38% compared to 10 μm/s.
  • Oocyte cleavage rates increased from 65.56% to 86.36% with increased penetration speed.
  • Significantly higher expression of totipotency and antiapoptotic genes was observed at 50 μm/s.

Conclusions:

  • Optimizing oocyte penetration speed based on intracellular strain is effective.
  • Higher penetration speeds (50 μm/s) enhance oocyte developmental potential and viability.
  • The optimized method shows positive effects at the gene expression level, confirming its efficacy.