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Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
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Microfluidic Immobilized Enzymatic Reactors for Proteomic Analyses-Recent Developments and Trends (2017-2021).

Cynthia Nagy1, Ruben Szabo1, Attila Gaspar1

  • 1Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem ter 1., 4032 Debrecen, Hungary.

Micromachines
|February 25, 2022
PubMed
Summary

Microfluidic immobilized enzyme reactors (μ-IMERs) are crucial for proteomics, offering enzyme reusability and faster sample analysis. This review covers recent trends in μ-IMER preparation and application for proteomic analysis.

Keywords:
2017–2021enzyme immobilizationenzyme reactormicrofluidicmonolithparticleprotein digestion

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Microfluidics

Background:

  • Microfluidic immobilized enzyme reactors (μ-IMERs) integrate physical, chemical, and engineering principles.
  • μ-IMERs are gaining importance in proteomics for enzyme reusability, reduced digestion times, and small sample volume handling.

Purpose of the Study:

  • To review current trends (2017-2021) in microfluidic immobilized enzyme reactor technology.
  • To survey microfabrication aspects (designs, fabrication, detectors) and enzyme immobilization strategies (supports, configurations).
  • To focus on μ-IMERs specifically developed for proteomic analysis.

Main Methods:

  • Literature review of μ-IMERs from 2017-2021.
  • Survey of microfabrication techniques and immobilization strategies.
  • Analysis of μ-IMER applications in proteomics.

Main Results:

  • Detailed overview of microdevice preparation and immobilization techniques.
  • Exploration of various IMER configurations and their suitability for proteomic applications.
  • Identification of advantages and limitations of different μ-IMER approaches.

Conclusions:

  • μ-IMERs offer significant advantages for proteomic workflows, including on-line integration potential.
  • The review provides a perspective on future directions for μ-IMER development in proteomics.