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Updated: Oct 2, 2025

A High-Throughput Platform for Culture and 3D Imaging of Organoids

A High-Throughput Platform for Culture and 3D Imaging of Organoids

Published on: October 14, 2022

Microscopic Imaging Methods for Organ-on-a-Chip Platforms.

Bailey C Buchanan¹, Jeong-Yeol Yoon¹

¹Department of Biomedical Engineering, The University of Arizona, Tucson, AZ 85721, USA.

|February 25, 2022

Summary

This summary is machine-generated.

Microscopic imaging is crucial for organ-on-a-chip (OOC) monitoring. This review details various imaging techniques applicable to OOCs, highlighting current limitations and future potential for enhanced biological insights.

Keywords:

OOC fluorescence microfluidic device smartphone-based microscopy transillumination

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Last Updated: Oct 2, 2025

A High-Throughput Platform for Culture and 3D Imaging of Organoids

A High-Throughput Platform for Culture and 3D Imaging of Organoids

Published on: October 14, 2022

Three-Dimensional Imaging of Organoids to Study Primary Ciliogenesis During ex vivo Organogenesis

Three-Dimensional Imaging of Organoids to Study Primary Ciliogenesis During ex vivo Organogenesis

Published on: May 14, 2021

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Published on: June 5, 2020

Area of Science:

Biomedical Engineering
Microscopy
Organ-on-a-Chip Technology

Background:

Microscopic imaging is the primary method for in situ monitoring of organ-on-a-chip (OOC) platforms.
OOCs require detailed cellular and molecular analysis for evaluating experimental outcomes.
Existing imaging methods for conventional microfluidics are not always optimized for OOC applications.

Purpose of the Study:

To provide a comprehensive review of microscopic imaging techniques for organ-on-a-chip platforms.
To compare the applicability of different imaging modalities to OOCs versus traditional microfluidic devices.
To identify specific challenges and future directions for OOC imaging.

Main Methods:

Review of transillumination imaging (brightfield, phase-contrast, holographic optofluidic).
Review of fluorescence imaging (confocal, light-sheet).
Review of smartphone-based imaging (microscope attachments, quantitative phase, lens-free).

Main Results:

Various imaging methods, including transillumination, fluorescence, and smartphone-based techniques, are applicable to OOCs.
Fewer imaging methods have been extensively demonstrated for OOCs compared to conventional microfluidics.
Specific imaging requirements for OOCs differ from those for standard microfluidic devices.

Conclusions:

Microscopic imaging is vital for OOC development and analysis.
Further research is needed to adapt and optimize imaging techniques for OOC platforms.
Addressing specific OOC imaging requirements will drive future advancements in the field.