Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deterministic, dynamically reconfigurable single quantum emitters enabled by tip-enhanced nano-optical trapping spectroscopy.

Nature communications·2026
Same author

Label-free optical observation of disordered-to-ordered transitions in single intrinsically disordered proteins.

Npj biosensing·2026
Same author

Design and synthesis of RNA vaccines encoding SAG1 antigen of toxoplasma gondii and evaluation of immunogenicity and its protective efficacy against acute toxoplasmosis in BALB/c mice.

Vaccine·2026
Same author

PD-1 and TIM-3 Expression on Peripheral Blood T Cells in HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP) and Their Correlations With Disease Stage and Proviral Load.

Journal of medical virology·2026
Same author

Small Molecule Activators of Protein Phosphatase 2A Exert Global Stabilizing Effects on the Scaffold PR65.

JACS Au·2026
Same author

Synthesis and characterization of silica nano particles-based imprinted polymers for detection of herpes simplex virus type 1 in human plasma.

Iranian journal of microbiology·2026

Related Experiment Video

Updated: Oct 2, 2025

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements
08:50

High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements

Published on: May 12, 2023

2.3K

Accessible high-performance double nanohole tweezers.

Ghazal Hajisalem, Elham Babaei, Michael Dobinson

    Optics Express
    |February 25, 2022
    PubMed
    Summary

    Researchers developed a new, accessible method for creating double nanohole (DNH) substrates for protein trapping using optical tweezers. This technique avoids complex top-down fabrication, enhancing protein analysis capabilities for scientists.

    More Related Videos

    Nanomanipulation of Single RNA Molecules by Optical Tweezers
    06:59

    Nanomanipulation of Single RNA Molecules by Optical Tweezers

    Published on: August 20, 2014

    15.0K
    Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
    06:53

    Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

    Published on: November 18, 2022

    2.3K

    Related Experiment Videos

    Last Updated: Oct 2, 2025

    High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements
    08:50

    High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements

    Published on: May 12, 2023

    2.3K
    Nanomanipulation of Single RNA Molecules by Optical Tweezers
    06:59

    Nanomanipulation of Single RNA Molecules by Optical Tweezers

    Published on: August 20, 2014

    15.0K
    Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
    06:53

    Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

    Published on: November 18, 2022

    2.3K

    Area of Science:

    • Nanotechnology
    • Biophysics
    • Optical physics

    Background:

    • Nanohole optical tweezers are established tools for protein analysis.
    • Existing methods for creating nanohole substrates often require complex top-down fabrication techniques.

    Purpose of the Study:

    • To develop a high-performance double nanohole (DNH) substrate fabrication method that bypasses top-down approaches.
    • To enable accessible and high-quality optical trapping of proteins for a wider scientific community.

    Main Methods:

    • Utilized polarization analysis for identifying and orienting DNHs.
    • Investigated substrate material changes, comparing glass to polyvinyl chloride (PVC).
    • Employed tape exfoliation for DNH exposure, avoiding damaging sonication or chemical treatments.

    Main Results:

    • Successfully created DNH substrates without top-down fabrication.
    • Identified various hole configurations, including single holes, trimers, and clusters.
    • Demonstrated a 7-fold reduction in minimum trapping power using PVC substrates, attributed to reduced surface repulsion.
    • Validated tape exfoliation as a non-damaging method for DNH exposure.

    Conclusions:

    • Developed an accessible, high-performance DNH substrate fabrication method.
    • Enhanced protein trapping efficiency through material modification (PVC).
    • Tape exfoliation offers a gentle and effective means for substrate preparation.