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Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
Published on: August 10, 2022
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Protein Expression Landscape Defines the Formation Potential of Mouse Blastoids From EPSCs.
Zheying Min1,2, Ke Zhong1, Yuxin Luo3
1Department of Obstetrics and Gynecology, Key Laboratory for Major Obstetric Diseases of Guangdong Province, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Frontiers in Cell and Developmental Biology
|February 25, 2022
Summary
Mouse blastoids, derived from extended pluripotent stem cells, mimic early embryonic development. Glucose metabolism is crucial for blastoid formation, as revealed by proteomic and phosphoproteomic analyses.
Area of Science:
- Developmental Biology
- Proteomics
- Metabolomics
Background:
- Preimplantation embryo development relies on maternal and newly synthesized proteins.
- Blastocyst-like structures, termed blastoids, can be generated from mouse embryonic stem cells (ESCs) or extended pluripotent stem cells (EPSCs).
Purpose of the Study:
- To investigate the dynamic protein and post-translational modification (PTM) expression in mouse EPS blastoids.
- To compare the proteomic and metabolomic profiles of EPS blastoids with early mouse embryos.
Main Methods:
- Quantitative mass spectrometry (MS) using a TMT-based strategy was employed.
- Phosphoproteomic analysis identified protein phosphorylation sites.
Main Results:
- The study revealed the comprehensive protein expression profile of EPS blastoids.
- Metabolite characteristics were analyzed, highlighting differences compared to early mouse embryos.
- Key proteins and phosphorylation sites involved in blastoid development were identified.
Conclusions:
- The proteomic profile of EPS blastoids closely resembles that of early mouse embryos.
- Glucose metabolism plays a critical role in the formation and development of blastoids.
