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Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

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Complex pBAE Nanoparticle Cell Trafficking: Tracking Both Position and Composition Using Super Resolution Microscopy.

Roger Riera¹, Jana Tauler^1,2, Natàlia Feiner-Gracia¹

¹Department of Biomedical Engineering, Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, De Zaale, Eindhoven, 5612 AZ (The, Netherlands.

|February 25, 2022

Summary

Direct stochastic optical reconstruction microscopy (dSTORM) tracks nanoscale materials. Oligopeptide modifications on poly(β-aminoester) nanoparticles influence their stability and cell trafficking, enabling rational design for nanomedicine.

Keywords:

cell trafficking direct stochastic optical reconstruction microscopy (dSTORM)nanoparticle stability poly(β-aminoester) nanoparticles

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Area of Science:

Nanomedicine
Biomaterials Science
Microscopy

Background:

Tracking nanoscale materials is crucial for nanomedicine but challenging due to their small size.
Conventional techniques often lack the resolution to study nanomaterials effectively.
Poly(β-aminoester) (pBAE) nanoparticles with specific oligopeptide modifications show promise for various biomedical applications.

Purpose of the Study:

To investigate the time stability and cellular trafficking of oligopeptide end-modified poly(β-aminoester) (OM-pBAE) nanoparticles.
To evaluate the impact of different cationic end oligopeptides (arginine, histidine, lysine) on nanoparticle behavior.
To establish a method for rational selection of pBAE nanoparticle compositions based on stability and cellular uptake.

Main Methods:

Utilized direct stochastic optical reconstruction microscopy (dSTORM) for high-resolution nanoscale imaging.
Synthesized and characterized OM-pBAE nanoparticles with varying oligopeptide modifications.
Studied nanoparticle time evolution, cell uptake, and intracellular trafficking in cellular models.

Main Results:

Demonstrated that the time stability and cell trafficking of OM-pBAE nanoparticles are significantly influenced by the specific oligopeptide modifications.
Identified distinct patterns of cellular uptake and intracellular routing dependent on the oligopeptide composition (arginine, histidine, lysine).
Showcased dSTORM as a powerful tool for analyzing nanoscale material behavior in biological systems.

Conclusions:

The choice of oligopeptide end-group critically affects the stability and cellular fate of pBAE nanoparticles.
dSTORM microscopy provides essential mechanistic insights into nanoparticle-cell interactions at the nanoscale.
This research facilitates the rational design of targeted pBAE nanoparticles for advanced nanomedicine applications.