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Chirality in Nature

Chirality in Nature

Chirality is the most intriguing yet essential facet of nature, governing life’s biochemical processes and precision. It can be observed from a snail shell pattern in a macroscopic world to an amino acid, the minutest building block of life. Most of the snails around the world have right-coiled shells because of the intrinsic chirality in their genes. All the amino acids present in the human body exist in an enantiomerically pure state, except for glycine - the sole achiral amino acid.

Prochirality

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The concept of prochirality leads to the nomenclature of the individual faces of a molecule and plays a crucial role in the enantioselective reaction. It is a concept where two or more achiral molecules react to produce chiral products. A typical process is the reaction of an achiral ketone to generate a chiral alcohol. Here, the achiral reactant reacts with an achiral reducing agent, sodium borohydride, to generate an equimolar mixture of the chiral enantiomers of the product. For example, an...

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Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates

Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates

Published on: March 5, 2019

Characterizing Aptamers with Reconfigurable Chiral Plasmonic Assemblies.

Yike Huang¹, Minh-Kha Nguyen^1,2,3, Vu Hoang Nguyen¹

¹Department of Neuroscience and Biomedical Engineering, School of Science, Aalto University, FI-00076 Aalto, Finland.

Langmuir : the ACS Journal of Surfaces and Colloids

|February 25, 2022

Summary

This summary is machine-generated.

This study introduces a new method using DNA origami and plasmonic assemblies to reliably measure aptamer binding affinities and specificities. This approach addresses inconsistencies, paving the way for advanced aptamer applications.

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Area of Science:

Biotechnology
Nanotechnology
Molecular Biology

Background:

Aptamers are DNA or RNA molecules that bind targets with high specificity, serving as alternatives to antibodies.
Inconsistent reported affinities and specificities of aptamers hinder their widespread application.
Reliable characterization methods are crucial for advancing aptamer-based technologies.

Purpose of the Study:

To develop a robust strategy for characterizing aptamer affinities and specificities.
To overcome the limitations of current aptamer characterization techniques.
To enable reproducible and accurate assessment of diverse aptamers.

Main Methods:

Utilized DNA origami-based chiral plasmonic assemblies as sensitive reporters.
Established a competitive hybridization reaction-based thermodynamic model for analysis.
Demonstrated the method on various DNA aptamers targeting small and large molecules.

Main Results:

Successfully characterized DNA aptamers with varying affinities (high and low).
Validated the effectiveness of the DNA origami-plasmonic assembly reporter system.
The thermodynamic model accurately reflected aptamer binding characteristics.

Conclusions:

The presented strategy offers a reliable and reproducible method for aptamer characterization.
This approach can be adapted for a broad range of aptamers.
Advancing aptamer characterization will accelerate the development of aptamer-based applications.