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Related Experiment Video

Updated: Oct 2, 2025

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection
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Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

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Hydrodynamic Injection for Developing NASH Model.

Haichuan Wang1, Xin Chen2

  • 1Department of Bioengineering and Therapeutic Sciences and Liver Center, University of California, San Francisco, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 25, 2022
PubMed
Summary
This summary is machine-generated.

Hydrodynamic tail vein injection (HTVi) effectively creates animal models for studying non-alcoholic steatohepatitis (NASH). This method facilitates gene delivery to the liver, aiding research into complex NASH mechanisms.

Keywords:
Hydrodynamic tail vein injectionMouseNonalcoholic steatohepatitisRat

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Area of Science:

  • Hepatology
  • Molecular Biology
  • Animal Models

Background:

  • Nonalcoholic steatohepatitis (NASH) is a growing health concern linked to obesity.
  • NASH involves liver inflammation and damage due to fat accumulation.
  • The molecular pathways underlying NASH pathogenesis are complex and not fully understood.

Purpose of the Study:

  • To describe the use of hydrodynamic tail vein injection (HTVi) for establishing animal models of NASH.
  • To provide a reliable method for studying liver diseases, specifically NASH.

Main Methods:

  • Hydrodynamic tail vein injection (HTVi) technique.
  • In vivo transfection of exogenous DNA into rodent livers.
  • Establishment of animal models for NASH research.

Main Results:

  • HTVi enables efficient gene delivery to the liver in vivo.
  • Successful establishment of animal models for NASH study.
  • Provides a foundation for investigating NASH molecular mechanisms.

Conclusions:

  • HTVi is a valuable tool for creating NASH animal models.
  • This technique supports research into the complex pathogenesis of NASH.
  • Further understanding of NASH molecular mechanisms can be achieved using these models.