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Related Experiment Video

Updated: Oct 2, 2025

Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport
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Bile Acid Profiling in Mouse Biofluids and Tissues.

Bo Kong1, Daniel Rizzolo1, Rulaiha E Taylor1

  • 1Department of Pharmacology and Toxicology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 25, 2022
PubMed
Summary

This study details a method for analyzing bile acid (BA) profiles in mice using HPLC-MS. Understanding individual BA changes is crucial for diagnosing non-alcoholic steatohepatitis (NASH) and related liver diseases.

Keywords:
Bile acid profilingFarnesoid X receptorHPLC-MSMouse tissues

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Area of Science:

  • Biochemistry
  • Metabolomics
  • Hepatology

Background:

  • Bile acids (BAs) are key signaling molecules regulating physiological and pathological processes.
  • Non-alcoholic steatohepatitis (NASH) involves impaired BA synthesis and metabolism due to liver injury and inflammation.
  • Altered BA composition can influence cell signaling pathways, contributing to NASH pathogenesis.

Purpose of the Study:

  • To present a method for analyzing individual bile acid profiles.
  • To facilitate research into the role of specific bile acids in disease development.

Main Methods:

  • High-performance liquid chromatography (HPLC) coupled with ion trap mass spectrometry (MS).
  • Analysis of individual bile acid profiles in mouse biofluids and tissues.

Main Results:

  • The study establishes a robust HPLC-MS method for precise bile acid profiling.
  • This method allows for the detailed analysis of individual bile acids in complex biological samples.

Conclusions:

  • Accurate analysis of individual bile acid profiles is essential for understanding their role in NASH.
  • The presented HPLC-MS method provides a valuable tool for NASH research and diagnostics.