A New Analytical Method to Quantify Ammonia in Freshwater with a Bulk Acoustic Wave Sensor

A new method to analyse ammonia in freshwater, based on a piezoelectric quartz crystal coated with the metalloporphyrin chloro[5,10,15,20-tetrakis(pentafluorophenyl)porphyrinato] manganese(III) is presented. A 9 MHz quartz crystal coated on both faces with an amount of porphyrin produced a frequency decrease of 21.4 kHz, which allowed ammonia in a 10.00 mL sample to be quantified in concentrations between 5 and 70 µg L^-1, with a sensitivity of 0.60 Hz L µg^-1, over a period of at least eight months. The proposed method has several advantages over the officially recommended indophenol spectrophotometric method: sample volume was reduced by a factor of 2.5, toxic reagents (phenol and sodium nitroprusside) were eliminated, analysing turbid samples presented no difficulty, and there was not only a significant time saving in solution preparation, but also in sample analysis time, which was reduced from 1 h to 2 min. No statistically significant differences (α = 0.05) were found both in the mean and precision of the results obtained for ammonia in water samples collected from domestic wells, analysed by this new method and by the indophenol spectrophotometric method. Furthermore, the proposed method would allow the individual quantification, with similar sensitivity, of amines and ammonia within a single analytical run.