Concentrically Encapsulated Dual-Enzyme Capsules for Synergistic Metabolic Disorder Redressing and Cytotoxic...

Area of Science:

• Biomaterials Science
• Nanotechnology
• Enzyme Engineering

Background:

• Enzyme therapy is crucial for metabolic disorders and detoxification.
• Challenges exist in creating efficient enzymatic nanoreactors with minimal toxic byproducts.
• Developing advanced nanocarriers is essential for improved therapeutic outcomes.

Purpose of the Study:

• To develop a novel strategy for preparing enzymes-loaded polypeptide microcapsules (EPM) with concentric enzyme encapsulation.
• To enhance cascade reaction rates and reduce cytotoxic intermediate emission in enzymatic nanoreactors.
• To investigate the efficacy of these EPMs in correcting glucose metabolism disturbances and clearing hydrogen peroxide.

Main Methods:

• Utilized mesoporous silica spheres (MSS) as a matrix for loading glucose oxidase (GOx).
• Employed layer-by-layer (LbL) assembly to immobilize catalase and polyelectrolytes on MSS.
• Developed a concentric encapsulation strategy, removing MSS to yield EPM with internal GOx and shell-immobilized catalase.

Main Results:

• Achieved efficient concentric encapsulation of enzymes within polypeptide microcapsules.
• Demonstrated 99% clearance of hydrogen peroxide (H₂O₂), a cytotoxic intermediate.
• Observed a doubled glucose consumption rate, indicating enhanced therapeutic efficiency.

Conclusions:

• The developed EPM strategy enables highly efficient dual-enzyme nanoreactors for metabolic disorder treatment.
• Concentric encapsulation minimizes cytotoxic byproduct release and boosts therapeutic enzyme activity.
• This approach shows significant promise for developing advanced enzymatic nanoreactors for various therapeutic applications.