Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Collagen processing in ras-transfected mouse mammary epithelial cells.

M J Warburton, S A Ferns, N E Hynes

    Biochemical and Biophysical Research Communications
    |May 29, 1986
    PubMed
    Summary

    Ras oncogene transformation in mouse mammary epithelial cells (NMuMG) disrupts collagen deposition, hindering basement membrane formation and promoting tumor cell invasion into surrounding tissues.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Phosphoinositide 3-kinase signaling is critical for ErbB3-driven breast cancer cell motility and metastasis.

    Oncogene·2011
    Same author

    Copine-III interacts with ErbB2 and promotes tumor cell migration.

    Oncogene·2009
    Same author

    A DNA delivery system targeting dendritic cells for use in immunization against malaria: a rodent model.

    Tanzania health research bulletin·2006
    Same author

    Internalisation enhances photo-induced cytotoxicity of monoclonal antibody-phthalocyanine conjugates.

    British journal of cancer·2001
    Same author

    The protein tyrosine phosphatase-PEST is implicated in the negative regulation of epidermal growth factor on PRL signaling in mammary epithelial cells.

    Molecular endocrinology (Baltimore, Md.)·2001
    Same author

    The substrate range of tripeptidyl-peptidase I.

    European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society·2001

    Area of Science:

    • Oncology
    • Cell Biology
    • Biochemistry

    Background:

    • The c-rasH oncogene is implicated in tumor formation.
    • Epithelial-stroma interactions are crucial for tissue structure and basement membrane deposition.

    Purpose of the Study:

    • To investigate the effect of ras oncogene transformation on the behavior of mouse mammary epithelial cells (NMuMG).
    • To determine how ras transformation impacts collagen synthesis and basement membrane deposition in vitro and in vivo.

    Main Methods:

    • Transfection of NMuMG cells with the c-rasH oncogene.
    • Culturing of NMuMG and NMuMG/p-rasH cells on plastic and collagen gels.
    • Quantification of collagen synthesis and deposition.

    Main Results:

    Related Experiment Videos

    • Ras-transformed NMuMG cells formed invasive tumors in nude mice.
    • Both cell types produced similar amounts of collagen on plastic.
    • NMuMG cells increased collagen synthesis on collagen gels, unlike ras-transformed cells.

    Conclusions:

    • Ras transformation partially inhibits epithelial-stroma interactions necessary for basement membrane deposition.
    • This inhibition may facilitate tumor cell invasion of the stroma.
    • Ras-mediated disruption of basement membrane integrity is a key mechanism in cancer progression.