Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

526
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
526
CRISPR01:59

CRISPR

53.3K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.3K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.6K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.6K
Other Glycolytic Pathways01:24

Other Glycolytic Pathways

281
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
281
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.2K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.2K
What is Genetic Engineering?00:49

What is Genetic Engineering?

75.9K
Overview
75.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of classical and quantal calculations of helium three-body recombination.

The Journal of chemical physics·2015
Same author

[Experimental study of human bone marrow mesenchymal stem cells on regulating the biological characteristics of gastric cancer cells].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2015
Same author

BuShenYiQi granule inhibits atopic dermatitis via improving central and skin Hypothalamic-Pituitary-Adrenal axis function.

PloS one·2015
Same author

Comparison of language cortex reorganization patterns between cerebral arteriovenous malformations and gliomas: a functional MRI study.

Journal of neurosurgery·2015
Same author

Synthesis and characterization of a new Inonotus obliquus polysaccharide-iron(III) complex.

International journal of biological macromolecules·2015
Same author

Porous graphene-based material as an efficient metal free catalyst for the oxidative dehydrogenation of ethylbenzene to styrene.

Chemical communications (Cambridge, England)·2015

Related Experiment Video

Updated: Oct 2, 2025

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
10:10

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

14.4K

CRISPR-based metabolic engineering in non-model microorganisms.

Liangyu Lu1, Xiaolin Shen1, Xinxiao Sun1

  • 1State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.

Current Opinion in Biotechnology
|February 26, 2022
PubMed
Summary
This summary is machine-generated.

CRISPR gene editing systems are revolutionizing the use of non-model microorganisms as cell factories. This review highlights advances in CRISPR tools for metabolic pathway engineering in bacteria, fungi, and cyanobacteria.

More Related Videos

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
13:31

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Published on: October 31, 2014

14.0K
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

22.3K

Related Experiment Videos

Last Updated: Oct 2, 2025

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production
10:10

Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

14.4K
Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
13:31

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Published on: October 31, 2014

14.0K
Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
11:27

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

22.3K

Area of Science:

  • Synthetic Biology
  • Microbial Biotechnology
  • Gene Editing Technologies

Background:

  • Non-model microorganisms offer unique metabolic capabilities for biosynthesis.
  • Limited genetic tools hinder the exploitation of these microbial metabolic potentials.
  • CRISPR systems have emerged as powerful tools for microbial genetic manipulation.

Purpose of the Study:

  • To review advances in CRISPR-mediated genetic modification systems for non-model microorganisms.
  • To highlight successful applications of CRISPR in metabolic pathway engineering.
  • To discuss challenges and future directions for CRISPR in non-model microbes.

Main Methods:

  • Literature review of CRISPR system development in non-model bacteria, fungi, and cyanobacteria.
  • Analysis of successful metabolic pathway engineering strategies using CRISPR-based genome editing.
  • Identification of current limitations and future prospects for CRISPR efficiency.

Main Results:

  • CRISPR systems have been successfully adapted for genetic modification in diverse non-model microorganisms.
  • CRISPR-based genome editing enables efficient metabolic pathway engineering for product biosynthesis.
  • Significant progress has been made in developing and applying CRISPR tools.

Conclusions:

  • CRISPR technology is crucial for unlocking the biosynthetic potential of non-model microorganisms.
  • Further improvements in editing efficiency are needed to overcome current barriers.
  • CRISPR holds great promise for advancing microbial cell factory applications.