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Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
Published on: January 3, 2015
Generation of Mt3 Homozygote murine ES cell lines via CRISPR/Cas9 technology
Huanxin Zhou1, Hongyan Sun1, Zhijiang Rong1
1College of Chemical and Biological Engineering, Taiyuan University of Science and Technology, No.66 Waliu Road, Taiyuan 030024, PR China.
Abstract:
Metallothioneins belong to a superfamily of intracellular metal-binding proteins, have bearing on almost all biochemical processes,include free radical scavenging, apoptosis, regulation of intracellular redox balance, and anti-inflammatory processes. By using a CRISPR/Cas 9 system, we generated Mt3-/- mES line. This cell line has contributed to further investigation of the functions of Mtt3 during early development, as well as a cell model for screening for metal-related detoxificationan and antioxidant response phenotype during stem cell differentiation.

