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A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

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NIRII-HDs: A Versatile Platform for Developing Activatable NIR-II Fluorogenic Probes for Reliable In Vivo Analyte

Zuojia Qin¹, Tian-Bing Ren¹, Huijie Zhou¹

¹State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.

Angewandte Chemie (International Ed. in English)

|February 26, 2022

Summary

Researchers developed a versatile new dye scaffold for second near-infrared (NIR-II) fluorescent probes. This innovation enables high-contrast in vivo imaging and disease detection, improving upon existing limitations in probe design.

Keywords:

Fluorescent Probes In Vivo Sensing Liver Injury Metastatic Tumors NIR-II Imaging

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Area of Science:

Chemical Biology
Biomedical Imaging
Organic Chemistry

Background:

Small-molecule probes emitting in the second near-infrared (NIR-II) window offer deep tissue penetration and high signal-to-noise ratios for in vivo imaging.
Current NIR-II probes lack versatile, stable, optically tunable groups, limiting their adaptability for diverse biological targets.

Purpose of the Study:

To design a novel, versatile dye scaffold (NIRII-HDs) for developing advanced NIR-II activatable fluorescent probes.
To synthesize and characterize NIRII-HD5, a specific dye within the NIRII-HDs scaffold, optimizing it for superior optical properties and stability.
To demonstrate the utility of NIRII-HD5 by creating targeted probes for reactive oxygen species (ROS), thiols, and enzymes.

Main Methods:

Development of the NIRII-HDs dye scaffold with tunable optical properties.
Synthesis and characterization of the NIRII-HD5 dye, assessing its pKa, stability, and NIR-II fluorescence brightness.
Design and synthesis of three target-specific activatable probes based on NIRII-HD5 for ROS, thiols, and enzymes.

Main Results:

The NIRII-HD5 dye exhibited excellent optical properties, including a suitable pKa, high stability, and strong NIR-II fluorescence.
Three novel activatable NIR-II probes were successfully developed using the NIRII-HD5 dye.
These probes enabled reliable in vivo imaging of various diseases in mouse models and accurate evaluation of liver tissue redox potential during injury.

Conclusions:

The NIRII-HDs scaffold, particularly NIRII-HD5, provides a versatile platform for developing high-performance NIR-II activatable fluorescent probes.
The developed probes demonstrate significant potential for sensitive and specific in vivo disease diagnosis and biological process monitoring.
This work overcomes limitations in current NIR-II probe design, paving the way for enhanced biomedical imaging applications.