Shewanella jiangmenensis sp. nov., isolated from aquaculture water

A Gram-stain-negative and facultatively anaerobic bacterial strain designated as JM162201^T was isolated from aquaculture water for farming Pacific white shrimp (Litopenaeus vannamei). The genome size of strain JM162201^T was 4,436,316 bp, and the genomic DNA G + C content was 55.0%. Phylogenetic analysis based on 16S rRNA gene sequences and genomes showed that strain JM162201^T belonged to the genus Shewanella and was closely related to Shewanella litorisediminis SMK1-12^T (97.1%), Shewanella khirikhana TH2012^T (97.0%), and Shewanella amazonensis SB2B^T (96.0%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain JM162201^T and three reference type strains were below the recognized thresholds of 95.0-96.0% (for ANI) and 70.0% (for dDDH) for species delineation. Growth occurred at 10-40 °C (optimum, 30 °C), at pH 4.0-10.0 (optimum, 7.0-8.0), and in 0-6.0% NaCl (w/v, optimum, 0-0.1%). The major cellular fatty acids of strain JM162201^T were summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c), C_17:1 ω8c, iso-C_15:0, C_16:0, and C_15:0. The predominant quinones were MK7, Q-7, and Q-8. The major polar lipids were phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). Based on the polyphasic taxonomical analyses, strain JM162201^T represents a novel species of the genus Shewanella, for which the name Shewanella jiangmenensis sp. nov. is proposed, with the type strain JM162201^T (= GDMCC 1.2006^T = KCTC 82340^T).