Influence of probiotics on water quality in intensified Litopenaeus vannamei ponds under minimum-water exchange

The effects of two probiotics on NH₃ degradation, as well as the magnetic field (21.56 m tesla) on the germination and proliferation of Bacillus spores, were studied in-vitro. Additionally, the effect of these probiotics on water quality maintenance in Litopenaeus vannamei holding ponds was investigated. For 180 min, NH₃ degradation was assessed as follows: Set 1: ammonia-free tap water with NH₃; Probiotic A (5 × 10¹⁰ viable Bacillus spores/g) with NH₃; Probiotic B (multi spp. 2 × 10⁹ CFU/g) with NH₃; and Set 2: same as set 1 with 30 mg L^-1 OM. The magnetic field was tested on Probiotic A (3.5 × 10⁷ CFU) for 36 h in triplicate. In the presence of organic matter, both probiotics degrade NH₃. The viable Bacillus count increased within 6 h of being exposed to the magnetic field, reaching its peak after 36 h. Firstly, fifteen ponds (250,000 PL/acre) were investigated, then 360 water samples were collected from the same corresponding pond for 8 weeks, and subjected to T1: control; T2: Probiotic A (0.007 g/m³/2 weeks); T3: Probiotic B (0.03 g/m³/2 weeks). Both probiotics with TVC and NH₃ demonstrated a negative correlation, on the other hand, they showed a significant (P ≤ 0.01) improvement in DO and pH. Overall, both probiotics were able to degrade NH₃ and the magnetic field (21.56 m tesla) was efficient to improve the germination and proliferation of Bacillus spores in-vitro. Probiotics were also effective for reducing TVC and NH₃ levels by increasing dissolved oxygen and pH in pond water.