The Dissemination of NDM-1 in Acinetobacter baumannii Strains
Na Liu1,2, Xin Zheng3,4, Qianhui Zhu3,4
1Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, Interdisciplinary Innovation Institute of Medicine and Engineering, Beihang University, Beijing, 100191, People's Republic of China.
Abstract:
Acinetobacter baumannii is a common pathogen in hospitals and usually causes bacteremia, pneumonia, meningitis, peritonitis and other diseases. Isolates carried NDM-1 gene can make several antibiotics such as carbapenems and other beta-lactams ineffective. Nowadays, the number of A. baumannii strains carrying NDM-1 has been climbing year by year in recent years. To characterise the transmission of NDM-1 in A. baumannii, we collected 2576 human-derived genomes of A. baumannii strains from NCBI database and found that 186 strains contained NDM-1 gene. The multi-locus sequence typing, phylogenetic tree, NDM-1 gene organization and the single nucleotide polymorphisms of NDM-1 were investigated. We hope that our work will provide a theoretical basis for the prevention of dissemination of NDM-1 in A. baumannii.
Insights
Acinetobacter baumannii strains carrying the NDM-1 gene are increasingly resistant to critical antibiotics like carbapenems. This study analyzes NDM-1 transmission in these hospital-acquired infections to inform prevention strategies.
Area of Science:
- Medical Microbiology
- Genomics
- Antimicrobial Resistance
Background:
- Acinetobacter baumannii is a significant hospital pathogen causing severe infections.
- The New Delhi metallo-beta-lactamase 1 (NDM-1) gene confers resistance to carbapenems and other beta-lactam antibiotics.
- Increasing prevalence of NDM-1-positive A. baumannii strains poses a major public health threat.
Purpose of the Study:
- To investigate the transmission characteristics of the NDM-1 gene within Acinetobacter baumannii populations.
- To analyze the genomic features associated with NDM-1 carriage in A. baumannii.
- To provide a foundation for preventing the spread of NDM-1 in clinical settings.
Main Methods:
- Bioinformatic analysis of 2576 human-derived Acinetobacter baumannii genomes from the NCBI database.
- Identification of strains carrying the NDM-1 gene (186 strains).
- Multi-locus sequence typing (MLST), phylogenetic analysis, investigation of NDM-1 gene organization, and single nucleotide polymorphism (SNP) analysis.
Main Results:
- Out of 2576 genomes, 186 Acinetobacter baumannii strains harbored the NDM-1 gene.
- Genomic analysis revealed specific patterns in NDM-1 gene organization and distribution.
- Phylogenetic analysis and SNP data provided insights into the evolutionary relationships and transmission pathways of NDM-1 positive strains.
Conclusions:
- The study characterizes the genomic landscape and transmission dynamics of NDM-1 in Acinetobacter baumannii.
- Findings offer crucial insights into the dissemination of carbapenem resistance mediated by NDM-1.
- This research provides a theoretical basis for developing effective strategies to control NDM-1 spread in healthcare environments.
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