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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Super-resolution Imaging of the Bacterial Division Machinery

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

Super-Resolution Radial Fluctuations (SRRF) Microscopy.

Jayme Salsman¹, Graham Dellaire^2,3

¹Department of Pathology, Dalhousie University, Halifax, NS, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|February 26, 2022

Summary

Super-resolution Radial Fluctuations (SRRF) imaging offers accessible super-resolution microscopy for researchers using common equipment. This method, detailed in the NanoJ-SRRF workflow, enables high-resolution imaging of cellular structures like chromatin.

Keywords:

Chromatin EMCCD SRRF Super-resolution sCMOS

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Last Updated: Oct 2, 2025

Super-resolution Imaging of the Bacterial Division Machinery

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

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Published on: July 6, 2019

Area of Science:

Cell Biology
Microscopy
Biophysics

Background:

Super-resolution microscopy techniques offer enhanced cellular visualization.
Traditional super-resolution methods often require specialized equipment and complex sample preparation.
Accessibility remains a barrier for widespread adoption in life science research.

Purpose of the Study:

To present a user-friendly workflow for Super-resolution Radial Fluctuations (SRRF) imaging.
To demonstrate the application of SRRF for imaging chromatin structure.
To promote the adoption of accessible super-resolution microscopy in research laboratories.

Main Methods:

Utilized common microscopy setups and open-source ImageJ plugins.
Employed the NanoJ-SRRF software for image acquisition and analysis.
Focused on standard fluorophores, fluorescent proteins, and routine experimental methods.

Main Results:

Successfully generated super-resolution images of chromatin using SRRF.
Demonstrated that SRRF does not necessitate specialized sample preparations or reagents.
Provided a step-by-step guide for implementing the SRRF workflow.

Conclusions:

SRRF imaging provides an accessible route to super-resolution microscopy for life scientists.
The NanoJ-SRRF software facilitates the routine application of this technique.
Increased accessibility of super-resolution imaging can significantly advance cellular imaging research.