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Silencing of circ-CDK14 suppresses osteosarcoma progression through the miR-198/E2F2 axis

Jun Liu1, Jianwen Zhao2, Guang Feng3

  • 1Department of Hand Surgery, The Second Hospital of Jilin University, Changchun, 130000, China.

Experimental Cell Research
|February 26, 2022
PubMed
Abstract

Insights

Circular RNA cyclin dependent kinase 14 (circ-CDK14) promotes osteosarcoma (OS) progression by targeting the miR-198/E2F2 pathway. Silencing circ-CDK14 inhibits OS cell growth and metastasis, suggesting it as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma (OS) is the most common primary bone cancer.
  • Circular RNAs (circRNAs) play a role in OS development.
  • The specific function of circRNA cyclin dependent kinase 14 (circ-CDK14) in OS is under investigation.

Purpose of the Study:

  • To investigate the role of circ-CDK14 in osteosarcoma progression.
  • To elucidate the underlying molecular mechanisms involving miR-198 and E2F2.
  • To evaluate circ-CDK14 as a potential therapeutic target for OS.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot to measure molecule levels.
  • Cell Counting-8 Kit (CCK-8), flow cytometry, and transwell assays to assess cell behavior.
  • Dual-luciferase reporter, RNA pull-down, and RNA immunoprecipitation (RIP) assays to confirm molecular interactions.
  • In vivo animal studies to evaluate therapeutic efficacy.

Main Results:

  • Circ-CDK14 was upregulated, while miR-198 was downregulated in OS tissues and cell lines.
  • Circ-CDK14 silencing inhibited OS cell viability, migration, invasion, and glycolysis, and promoted apoptosis.
  • Circ-CDK14 directly targeted miR-198, which in turn targeted E2F2, forming a circ-CDK14/miR-198/E2F2 axis.
  • Circ-CDK14 knockdown suppressed tumor growth in vivo.

Conclusions:

  • Circ-CDK14 promotes osteosarcoma progression through the miR-198/E2F2 pathway.
  • Silencing circ-CDK14 exhibits inhibitory effects on OS.
  • Circ-CDK14 represents a promising novel therapeutic strategy for osteosarcoma.
Keywords:
E2F2GlycolysisOsteosarcoma (OS)circ-CDK14miR-198

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