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Copper mediated molecularly imprinted polymers for fast recognizing tylosin.

Yi Zhang1, Zhaoju Wu1, Haizhu Shi1

  • 1Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs, School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.

Journal of Pharmaceutical and Biomedical Analysis
|February 26, 2022
PubMed
Summary

Researchers developed novel magnetic molecularly imprinted polymers (MIPs) for rapid tylosin (TYL) antibiotic detection. These polymers efficiently extract TYL from complex samples like human urine, aiding in high-throughput analysis.

Keywords:
Copper mediated molecularly imprinted polymersHPLC/UV-VisHuman urineMagnetic microspheresTylosin

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biomedical Engineering

Background:

  • Molecularly imprinted polymers (MIPs) serve as artificial antibodies for selective molecule separation.
  • Tylosin (TYL) is an important macrolide antibiotic requiring sensitive detection methods.
  • Existing methods for TYL detection can be limited by complex sample matrices and analysis time.

Purpose of the Study:

  • To develop a novel copper-mediated magnetic MIP for efficient tylosin (TYL) extraction.
  • To evaluate the performance of the developed MIP for trace TYL residue analysis in human urine.
  • To establish a rapid and selective method for high-throughput analysis of TYL.

Main Methods:

  • Synthesis of copper-mediated magnetic MIPs with a superparamagnetic and hydrophilic surface.
  • Characterization of the MIP microspheres' morphology, hydrophilicity, and magnetic properties.
  • Application of the MIPs as a magnetic dispersed solid-phase extraction (MD-SPE) material for TYL enrichment.
  • Quantification of TYL using High-Performance Liquid Chromatography with UV-Vis detection (HPLC/UV-Vis).

Main Results:

  • The synthesized MIP microspheres exhibited a unique surface morphology with uniform convexities.
  • The material demonstrated high hydrophilicity, superparamagnetic behavior, and excellent selectivity for TYL.
  • Absorption equilibrium for TYL was reached rapidly within 5 minutes, enabling high-throughput analysis.
  • Mean recoveries for TYL in spiked human urine ranged from 76.42% to 93.72% with low relative standard deviations (2.75-8.24%).

Conclusions:

  • The novel copper-mediated magnetic MIPs offer a promising material for selective and efficient enrichment of tylosin.
  • The developed magnetic dispersed solid-phase extraction method is highly feasible for rapid, high-throughput analysis of trace TYL in complex matrices like human urine.
  • This work provides a valuable reference for designing advanced solid-phase extraction materials for trace component analysis.