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Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

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Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
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Assembly of Cytoskeletal Filaments01:18

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Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
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Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

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Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
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Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

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Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
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Updated: Oct 2, 2025

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
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Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

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Protein assembly and crowding simulations.

Lim Heo1, Yuji Sugita2, Michael Feig3

  • 1Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA. Electronic address: https://twitter.com/huhlim.

Current Opinion in Structural Biology
|February 26, 2022
PubMed
Summary
This summary is machine-generated.

Computer simulations reveal how molecular crowding and protein assembly impact biological functions. This review explores recent findings on protein folding, enzyme activity, and cellular interactions, highlighting simulation challenges.

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Area of Science:

  • Biophysics
  • Computational Biology
  • Biochemistry

Background:

  • Biological systems involve frequent protein molecular interactions.
  • Computer simulations offer mechanistic insights into in vivo protein interactions and biological functions.

Purpose of the Study:

  • To review recent computer simulations of protein assembly and molecular crowding.
  • To elucidate how these phenomena influence protein behavior and cellular functions.

Main Methods:

  • Focus on simulation studies of protein assembly pathways (e.g., virus capsids, amyloid aggregation).
  • Analysis of molecular crowding effects on protein folding, stability, enzyme activity, and diffusion.
  • Discussion of simulation techniques and challenges for modeling cellular environments.

Main Results:

  • Crowding simulations provide insights into protein folding, stability, enzyme modulation, and diffusion.
  • Assembly simulations explore pathways for virus capsids, amyloid formation, and phase separation driven by multivalent interactions.

Conclusions:

  • Computer simulations are crucial for understanding complex protein interactions in biological contexts.
  • Advances in simulation methods are needed for greater realism in modeling cellular environments.