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Updated: Oct 2, 2025
12:24
Quantifying Yeast Chronological Life Span by Outgrowth of Aged Cells
Published on: May 6, 2009
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Increased peroxisome proliferation is associated with early yeast replicative ageing
Rachayeeta Deb1, Suchetana Ghose1, Shirisha Nagotu2
1Organelle Biology and Cellular Ageing Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, India.
Current Genetics
|February 27, 2022
Summary
Cellular aging in yeast shows an increase in peroxisome number, particularly in aged mother cells. This peroxisome proliferation is linked to replicative age and influenced by specific fission proteins.
Area of Science:
- Cell Biology
- Aging Research
- Organelle Biology
Background:
- Peroxisomes are vital single-membrane organelles involved in cellular functions and reactive oxygen species metabolism.
- Their role in cellular aging is an active area of research across various model systems.
Purpose of the Study:
- To investigate changes in peroxisome number during early replicative aging in yeast cells.
- To determine if increased peroxisome numbers in aged cells result from enhanced proliferation and the involvement of specific proteins.
Main Methods:
- Culturing wild-type and mutant yeast strains (pex11, pex25, pex11pex25) under peroxisome-inducing and non-inducing conditions.
- Quantifying peroxisome numbers in young and aged mother cells.
- Analyzing peroxisome proliferation and mitochondrial morphology.
Main Results:
- Replicatively aged yeast mother cells exhibited an increased number of peroxisomes.
- Peroxisome proliferation was enhanced in aged cells, especially in mutants lacking Pex25, when cultured in oleic acid.
- Aged cells with more peroxisomes showed mitochondrial dysfunction and fragmentation.
Conclusions:
- A correlation exists between increased peroxisome number and replicative age in yeast.
- Peroxisome proliferation in aged cells is partly dependent on peroxisome fission proteins like Pex11 and Pex25.
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