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Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality
Published on: May 5, 2022
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Challenges to, and prospects for, reverse engineering the gastrointestinal tract using organoids
Panagiota Kakni1, Roman Truckenmüller1, Pamela Habibović1
1Department of Instructive Biomaterials Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229, ER, Maastricht, The Netherlands.
Trends in Biotechnology
|February 28, 2022
Summary
Organoid models of the digestive system are advancing. Future gastrointestinal tract (GIT) organoids aim to integrate multiple regions into single in vitro models for comprehensive study.
Area of Science:
- Gastroenterology and Organoid Technology
- In Vitro Modeling
- Digestive System Research
Background:
- Organoids modeling the digestive system have been studied for over a decade.
- Creating organoid models for all gastrointestinal tract (GIT) regions is challenging due to protocol variations.
- Existing models often require specific modifications for each tissue surrogate.
Purpose of the Study:
- To review challenges and advances in gastrointestinal tract (GIT) organoid models.
- To discuss the development of next-generation integrated GIT organoids.
- To provide an outlook on the future of in vitro models for the digestive system.
Main Methods:
- Review of current literature on GIT organoid development.
- Analysis of challenges in recapitulating diverse GIT regions.
- Discussion of emerging trends in integrated organoid systems.
Main Results:
- Significant progress has been made in developing organoid models for various GIT regions.
- Tailor-made protocols are necessary for generating specific intestinal organoids.
- Integrated organoid models are emerging as a next-generation approach.
Conclusions:
- GIT organoid technology has advanced significantly but faces challenges in comprehensive regional recapitulation.
- Integrated organoid models hold promise for mimicking multiple digestive system regions in one system.
- The future of GIT in vitro models involves more complex, integrated systems for advanced research.
