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Updated: Oct 2, 2025
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
Published on: June 27, 2017
Co-delivery of genes can be confounded by bicistronic vector design
Hanieh Moradian1,2,3, Manfred Gossen1,2, Andreas Lendlein1,2,3
1Institute of Active Polymers, Helmholtz-Zentrum Hereon, 14513 Teltow, Germany.
Abstract:
Maximizing the efficiency of nanocarrier-mediated co-delivery of genes for co-expression in the same cell is critical for many applications. Strategies to maximize co-delivery of nucleic acids (NA) focused largely on carrier systems, with little attention towards payload composition itself. Here, we investigated the effects of different payload designs: co-delivery of two individual "monocistronic" NAs versus a single bicistronic NA comprising two genes separated by a 2A self-cleavage site. Unexpectedly, co-delivery via the monocistronic design resulted in a higher percentage of co-expressing cells, while predictive co-expression via the bicistronic design remained elusive. Our results will aid the application-dependent selection of the optimal methodology for co-delivery of genes.
Supplementary Information:
The online version contains supplementary material available at 10.1557/s43579-021-00128-7.
