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Related Experiment Video

Updated: Oct 2, 2025

Reusable Single Cell for Iterative Epigenomic Analyses

Reusable Single Cell for Iterative Epigenomic Analyses

Published on: February 11, 2022

Reusable Single Cell for Iterative Epigenomic Analyses.

Hidetaka Ohnuki¹, David J Venzon², Alexei Lobanov³

¹Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health; hidetaka.ohnuki@nih.gov.

Journal of Visualized Experiments : Jove

|February 28, 2022

Summary

This study introduces a novel method for reusing single cells in epigenomic analyses, enabling iterative experiments. This approach enhances data density from rare cells and allows for more robust statistical analysis.

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Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

Area of Science:

Molecular Biology
Genomics
Epigenetics

Background:

Current single-cell epigenome analyses are destructive, limiting multi-omic analysis per cell.
Analyzing multiple epigenetic marks in a single cell is challenging due to cell destruction after initial use.

Purpose of the Study:

To develop a method for iterative epigenomic analyses on the same single cell.
To increase data density and enable robust statistical analysis from rare cells.

Main Methods:

Cells are anchored to a polyacrylamide polymer, preserving structure for reuse.
Genomic DNA is prepared for proximity ligation with antibody-bound DNA probes.
Iterative proximity ligation assays are performed on the same single cell.

Main Results:

Reusable single cells can be stored for months and analyzed multiple times.
The method allows for increased data density from rare cells.
Enables statistical analysis using only data from the same cell.

Conclusions:

This technique provides flexibility for researchers performing epigenomic studies.
Facilitates comprehensive epigenetic characterization of individual cells.
Overcomes limitations of single-use single-cell analysis.