C₃Mab-2: An Anti-Mouse CCR3 Monoclonal Antibody for Immunocytochemistry

The C-C motif chemokine receptor 3 (CCR3) is a G protein-coupled receptor activated by eotaxin-1-3, MCP-2-4, and RANTES. CCR3 is associated with allergic diseases and cancer development and is highly expressed in eosinophils, basophils, and cancer cells. Besides, research on the physiological roles of CCR3 is ongoing. Thus, specific monoclonal antibodies (mAbs) for CCR3 would be useful for diagnostic and therapeutic purposes and for unraveling the function of CCR3. We previously developed an anti-mouse CCR3 (mCCR3) mAb (C₃Mab-2; rat IgG_2b, kappa) using the Cell-Based Immunization and Screening method and showed that C₃Mab-2 could detect endogenous and exogenous mCCR3 in flow cytometry. In this study, we showed that C₃Mab-2 and its recombinant antibody (recC₃Mab-2f) specifically recognized endogenous mCCR3 in P388 (a mouse lymphocyte-like cell line) and J774-1 (a mouse macrophage-like cell line) cells and are usable in immunocytochemistry.