Salinarimonas soli sp. nov., isolated from soil

Area of Science:

• Microbiology
• Bacteriology
• Genomics

Background:

• Soil ecosystems harbor diverse microbial communities.
• Accurate bacterial classification is crucial for understanding ecological roles and potential applications.

Purpose of the Study:

• To isolate and characterize a novel bacterium from a Korean soil sample.
• To determine the phylogenetic and taxonomic position of the novel bacterium.

Main Methods:

• Isolation and cultivation of bacteria from soil.
• Gram staining, motility tests, and morphology observation.
• 16S rRNA gene sequencing for phylogenetic analysis.
• Chemotaxonomic analysis (polar lipids, fatty acids, ubiquinone).
• Genomic DNA G+C content determination.
• Genomic orthoANI analysis.
• CRISPR-Cas system identification.

Main Results:

• A light pink, Gram-negative, aerobic, motile, rod-shaped bacterium (strain BN140002^T) was isolated.
• Phylogenetic analysis indicated it belongs to the genus Salinarimonas, with similarities to S. rosea and S. ramus.
• Chemotaxonomic data (major polar lipids, fatty acids, Q-10 ubiquinone) and genomic G+C content (70.1 mol%) were determined.
• Genomic orthoANI values supported its distinctness from related species.
• A class I-C type CRISPR-Cas system was identified.

Conclusions:

• Strain BN140002^T represents a novel species within the genus Salinarimonas.
• The proposed name for this new species is Salinarimonas soli sp. nov.
• The type strain is BN140002^T (=KCTC 42643^T=CCTCC AB 2017173^T).