Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Packaging

DNA Packaging

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Glioma-derived extracellular vesicles as drivers of immunotherapeutic resistance: mechanisms of immune reprogramming and metabolic intervention.

Frontiers in immunology·2026

Same author

Emerging Poly(ethylene glycol)-Lipid Alternatives for New-Generation Lipid Nanoparticles.

Polymer science & technology (Washington, D.C.)·2026

Same author

Photoresponsive Discoidal Polymersomes With Tracelessly Crosslinkable Bilayers for Intracellular Drug Delivery.

Angewandte Chemie (International ed. in English)·2026

Same author

Dexime: A Selectively Enzyme-Degradable Hydrogel for Protein Therapeutic Release.

Advanced materials (Deerfield Beach, Fla.)·2026

Same author

Linking tumor viability and immune infiltration with dual-nucleus MRI in preclinical models.

Npj imaging·2026

Same author

Precision Oligomeric Building Blocks toward Chemically Recyclable Polyolefin-like Materials.

Precision chemistry·2026

Same journal

Fiber and continuum scale contributions to the intrinsic and apparent fracture of soft collagenous tissue <i>via</i> cutting.

Biomaterials science·2026

Same journal

Surface morphology-regulated tissue adhesion in solid and mesoporous silica-reinforced gelatin nanocomposite hydrogels.

Biomaterials science·2026

Same journal

Nanostructured hyaluronic acid-chia mucilage film as bioactive wound dressings for accelerated skin regeneration.

Biomaterials science·2026

Same journal

Tunable bio-inspired hybrid hydrogels reprogram stem cell-derived extracellular vesicles for superior wound regeneration.

Biomaterials science·2026

Same journal

Bioorthogonally reinforced injectable granular hydrogels synergizing ECM mimicry with microporosity for skin tissue engineering.

Biomaterials science·2026

Same journal

Modeling a hypoxia-integrated glioblastoma microenvironment to mimic tumor heterogeneity and chemoresistance.

Biomaterials science·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 2, 2025

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

Self-immolative polyplexes for DNA delivery.

Quinton E A Sirianni¹, TianDuo Wang^2,3, Aneta Borecki¹

¹Department of Chemistry and the Centre for Advanced Materials and Biomaterials Research, The University of Western Ontario, 1151 Richmond St, London, Ontario, N6A 5B7, Canada. egillie@uwo.ca.

Biomaterials Science

|February 28, 2022

Summary

This summary is machine-generated.

This study introduces a novel self-immolative polyglyoxylamide (PGAm) platform for enhanced nucleic acid delivery. These polymers effectively deliver genetic material into cells and reduce cytotoxicity by depolymerizing under acidic conditions.

More Related Videos

Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery

Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery

Published on: August 6, 2019

Assembly and Characterization of Polyelectrolyte Complex Micelles

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Related Experiment Videos

Last Updated: Oct 2, 2025

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery

Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery

Published on: August 6, 2019

Assembly and Characterization of Polyelectrolyte Complex Micelles

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

Area of Science:

Biomaterials Science
Polymer Chemistry
Gene Therapy

Background:

Nucleic acid therapies require efficient and safe delivery systems.
Current polycationic delivery vehicles face challenges balancing cytotoxicity and efficacy.
Polycations complex with nucleic acids but often exhibit significant toxicity.

Purpose of the Study:

To develop a novel self-immolative polymer platform for nucleic acid delivery.
To create a system that releases nucleic acids upon depolymerization, reducing cellular toxicity.
To investigate the relationship between polymer structure, depolymerization, and transfection efficiency.

Main Methods:

Synthesis and characterization of nine polyglyoxylamide (PGAm) variants with varying end-caps and cationic groups.
Complexation of PGAm with plasmid DNA to form nanoparticles.
Assessment of PGAm depolymerization rates under mildly acidic conditions.
In vitro cytotoxicity assays using HEK 293T cells.
Transfection efficiency assays measuring reporter gene expression.

Main Results:

Synthesized PGAm polymers effectively complexed plasmid DNA into nanoparticles.
PGAm depolymerization occurred under mildly acidic conditions, releasing the nucleic acid cargo.
Depolymerization led to reduced cytotoxicity of the PGAm/DNA complexes.
Selected PGAm analogues demonstrated comparable transfection efficiency to commercial agents with lower toxicity.
A specific PGAm analogue showed promising activity dependent on depolymerization and low cytotoxicity.

Conclusions:

Self-immolative polyglyoxylamides offer a promising new platform for nucleic acid delivery.
End-to-end depolymerization is a viable strategy to mitigate polycation cytotoxicity.
These degradable polymers represent a significant advancement in developing safer gene delivery vehicles.