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Design of a DNAzyme : Prediction of mRNA Regions Accessible to a DNAzyme
Gerhard Steger1, Julian Victor2
1Institut für Physikalische Biologie, Heinrich-Heine-Universität Düsseldorf, Düsseldorf, Germany. steger@biophys.uni-duesseldorf.de.
Methods in Molecular Biology (Clifton, N.J.)
|February 28, 2022
Abstract:
The efficiency of RNA-cleaving DNAzymes depends on a large extent on complex formation with their RNA targets. We describe available prediction tools that should help in the design of efficient DNAzymes and show some experimental methods to test the predictions. The main example is for a 10-23 DNAzyme, but the procedure works as well for the 8-17 DNAzyme family.

