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Enhancement of Activity for Peroxidase-Mimicking DNAzyme by Covalent Attachment of Hemin to G-Quadruplex-Forming
1Department of Bioanalytical Chemistry, Faculty of Chemistry, Adam Mickiewicz University, Poznań, Poland. kosman@amu.edu.pl.
Abstract:
Peroxidase-mimicking DNAzymes are used in the development of new bioanalytical assays due to their advantages like thermal stability, simple synthesis and purification, and ability to hybridize with the complementary strand of nucleic acid. Here, we describe the method of covalent attachment of hemin to DNA oligonucleotide using click chemistry that allows good yield (60-70%) of the final conjugate product. The activity of obtained DNAzymes is monitored using chromogenic and fluorogenic substrates.
