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Cell surface carbohydrate changes during embryonic and fetal skin development.

E Dabelsteen, K Holbrook, H Clausen

    The Journal of Investigative Dermatology
    |July 1, 1986
    PubMed
    Summary
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    During embryonic development, N-acetyllactosamine is abundant in skin but later modified into blood group antigens H, Lex, and Ley. These changes reflect cell differentiation processes.

    Area of Science:

    • Developmental Biology
    • Carbohydrate Chemistry
    • Immunohistochemistry

    Background:

    • Type 2 chain carbohydrate antigens play roles in cellular processes.
    • Understanding their expression during human development is crucial.

    Purpose of the Study:

    • To investigate the expression patterns of N-acetyllactosamine, blood group antigen H, Lex, and Ley in embryonic and fetal skin.
    • To correlate these patterns with skin differentiation stages.

    Main Methods:

    • Immunohistochemical analysis using monoclonal antibodies against four type 2 chain carbohydrate antigens.
    • Study of embryonic and fetal skin samples across different gestational ages.

    Main Results:

    • N-acetyllactosamine, Lex, and Ley were consistently found in the periderm.

    Related Experiment Videos

  • Blood group antigen H expression was transient in the periderm.
  • Epidermal expression of N-acetyllactosamine decreased with age, potentially masked by sialic acid.
  • Blood group antigen H appeared in the epidermis later in development.
  • Lex expression was observed in basal and intermediate cells, while Ley showed weak expression in intermediate cells.

    • Conclusions:

    • N-acetyllactosamine is maximally expressed in early skin development and subsequently modified.
    • Sialylation and fucosylation lead to the formation of blood group antigens H, Lex, and Ley during differentiation.
    • Observed changes in cell surface carbohydrates align with known patterns of chemical modifications during skin differentiation.