Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

2.6K
Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
2.6K
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

2.8K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
2.8K
Catenins01:23

Catenins

2.5K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
2.5K
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

2.9K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
2.9K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

5.3K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.3K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.9K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Towards globally equitable bioinformatics adoption.

PLoS biology·2026
Same author

MULTIDIMENSIONAL SPATIAL MAPPING OF EXTRACELLULAR MATRIX: CARTILAGINOUS-OSSEOUS COMPOSITE FORMATION, TENDON INTEGRATION AND VASCULARIZATION DURING SKELETAL GROWTH AND REPAIR.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2026
Same author

Heterogeneous Systemic IgG Responses to Porphyromonas gingivalis Gingipains in Advanced Periodontitis.

Clinical and experimental dental research·2026
Same author

Novel Netrin-1-Unc5b-Klf4 axis balances AT2 survival in experimental bronchopulmonary dysplasia.

American journal of respiratory cell and molecular biology·2026
Same author

Tenascin-C orchestrates radiotherapy-induced head and neck tumor regression.

EMBO molecular medicine·2026
Same author

ActIIR inhibition improves motor outcome and preserves muscle fibers after experimental autoimmune neuritis.

Acta neuropathologica communications·2026

Related Experiment Video

Updated: Oct 2, 2025

TGF-β-mediated Endothelial to Mesenchymal Transition EndMT and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing
07:05

TGF-β-mediated Endothelial to Mesenchymal Transition EndMT and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing

Published on: February 26, 2021

5.3K

Modulating tenascin-C functions by targeting the MAtrix REgulating MOtif, "MAREMO".

Thomas Loustau1, Chérine Abou-Faycal1, William Erne1

  • 1University Strasbourg, INSERM U1109, MN3T (The Microenvironmental Niche in Tumorigenesis and Targeted Therapy), 3 avenue Molière, Strasbourg, Hautepierre, France; University Strasbourg, INSERM U1109, The Tumor Microenvironment Laboratory, Hôpital Civil, Institut d'Hématologie et d'Immunologie, Fédération de Médecine Translationnelle de Strasbourg (FMTS), 1 Place de l'Hôpital, 67091 Strasbourg, France.

Matrix Biology : Journal of the International Society for Matrix Biology
|March 1, 2022
PubMed
Summary

Researchers identified a conserved M-motif in Tenascin-C (TNC) and fibronectin (FN) that mediates their interactions. Mimicking this motif with peptides blocked TNC functions, offering new strategies to inhibit TNC in cancer and fibrosis.

Keywords:
CCL21CXCL12Cell adhesionChemoretentionFibronectinGene expressionImmune suppressionMatrisomeMatrix Regulating Motif MAREMOMatrix assemblyProteomicsSignalingStructural modelingTGFβTRAILTenascin-C

More Related Videos

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
07:49

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Published on: August 3, 2018

11.4K
Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos
12:35

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

Published on: April 14, 2023

1.5K

Related Experiment Videos

Last Updated: Oct 2, 2025

TGF-β-mediated Endothelial to Mesenchymal Transition EndMT and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing
07:05

TGF-β-mediated Endothelial to Mesenchymal Transition EndMT and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing

Published on: February 26, 2021

5.3K
Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
07:49

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Published on: August 3, 2018

11.4K
Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos
12:35

Author Spotlight: Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

Published on: April 14, 2023

1.5K

Area of Science:

  • Extracellular matrix biology
  • Molecular oncology
  • Immunology

Background:

  • Tenascin-C (TNC) promotes cancer and inflammation by creating an immune-suppressive tumor microenvironment.
  • TNC interacts with fibronectin (FN) and other molecules, suggesting a common binding mechanism.
  • Understanding TNC's binding interactions is crucial for developing targeted therapies.

Purpose of the Study:

  • To identify a common binding mechanism between TNC and FN.
  • To investigate the role of a conserved motif in TNC-FN interactions.
  • To develop novel therapeutic strategies targeting TNC functions.

Main Methods:

  • Sequence comparison of FN domains (FN5, FN13) to identify conserved motifs.
  • Structural modeling and functional analysis to identify M-motifs in TNC.
  • Negative staining electron microscopy to visualize M-motif interactions.
  • Generation of M-motif mimetic peptides (P5, P13) and assessment of their inhibitory effects.

Main Results:

  • A conserved M-motif (MAREMO) was identified in FN and TNC.
  • The M-motif in FN mediates interactions with both FN and TNC.
  • M-motif mimetic peptides blocked TNC-FN binding, TNC-mediated cell rounding, FN matrix assembly, TNC signaling, and chemokine binding.
  • These peptides inhibited dendritic cell retention in the tumor microenvironment.

Conclusions:

  • The MAREMO/MBS interaction is a key mediator of TNC functions.
  • Targeting this interaction offers a novel strategy to inhibit TNC in cancer, inflammation, and fibrosis.
  • M-motif mimetic peptides represent a promising therapeutic approach.