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Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis

Meabh Cullinane1, Lauren Scofield2, Gerald L Murray2,3,4

  • 1Judith Lumley Centre, School of Nursing and Midwifery, La Trobe University, Melbourne, Victoria, 3086, Australia.

The Australian & New Zealand Journal of Obstetrics & Gynaecology
|March 1, 2022
PubMed

Mastitis is commonly experienced by breastfeeding women. While Staphylococcus aureus is usually implicated in infectious mastitis, coagulase-negative staphylococci (CoNS) are a possible alternative pathogen. This case-control study examined the role of CoNS in mastitis using isolates cultured from breast milk of 20 women with mastitis and 16 women without mastitis. Gene sequencing determined bacterial species, and random amplified polymorphic DNA (RAPD) analysis investigated strain-level variation. The majority of CoNS isolates were Staphylococcus epidermidis (182/199; 91%). RAPD analysis identified 33 unique S. epidermidis profiles, with no specific profile associated with mastitis cases.

Keywords:
Staphylococcus epidermidiscase-control studycoagulase-negative staphylococcimastitis

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