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Updated: Oct 1, 2025
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In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
Published on: July 8, 2020
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Parameters to identify good quality oocytes and embryos in cattle
1Chair for Molecular Reproductive Medicine, Clinic for Veterinary Obstetrics, Gynecology and Andrology of Large and Small Animals, Justus-Liebig-University Giessen, Frankfurter Straße 106, Giessen 35392, Germany.
Reproduction, Fertility, and Development
|March 1, 2022
Summary
Selecting high-quality oocytes and embryos is crucial for successful in vitro production (IVP) and embryo transfer. This review examines current and emerging methods for assessing embryo developmental competence, focusing on cattle.
Area of Science:
- Reproductive Biology
- Embryology
- Animal Science
Background:
- Oocyte and embryo selection methods are vital for assisted reproductive technologies.
- Current methods include invasive and non-invasive techniques applied from oocyte to blastocyst stages.
- Morphology and blastocyst development rate are key indicators of developmental competence.
Purpose of the Study:
- To review existing and novel methods for assessing oocyte and preimplantation embryo quality.
- To highlight the need for improved, non-invasive selection procedures.
- To focus on cattle as a model species.
Main Methods:
- Morphological assessment (including staining techniques).
- Molecular analyses (transcriptomics, proteomics).
- Metabolic profiling.
- Artificial intelligence applications.
Main Results:
- Morphological evaluation is the current standard for selecting viable oocytes and embryos.
- Invasive methods have aided in identifying quality-related genes.
- Non-invasive approaches are advancing but require further refinement.
- A combination of methods, including AI, shows promise for enhanced selection.
Conclusions:
- Further refinement of oocyte and embryo selection methods is necessary.
- Development of robust, non-invasive techniques is critical for selecting embryos with the highest developmental potential.
- Integrating advanced technologies like AI can improve embryo selection accuracy.
