In vivo metabolism of apolipoprotein E in humans

A method for the investigation of the in vivo metabolism of apoE in humans has been described. In this method, isolated apoE is radioiodinated by the iodine monochloride method and reassociated with lipoproteins. Detailed studies have established that the radiolabeled apoE prepared by this procedure uniformly labels the different plasma lipoprotein pools. The study subjects are studied in steady state on a dietary regimen with multiple feedings. The kinetic results are analyzed by either multiexponential curve fitting or by computer-assisted multicompartmental modeling techniques. Examples of the types of results that may be obtained utilizing these methods are described.