Research progress in the Helicobacter pylori with viable non-culturable state

Helicobacter pylori (H. pylori) is one of the most common pathogens in human beings and it is responsible for diseases such as chronic gastritis, peptic ulcer, and even gastric cancer. Studies in recent years have found that H. Pylori could transform from the normal spiral-shaped bacillary form into the coccoid form and enter a viable but non-culturable (VBNC) state, which may pose a potential threat to public health. In this state,the morphological structure and physiological characteristics of H. Pylori have changed. It can maintain the metabolic activity but protein expression is decreased. And the H. Pylori in this state cannot grow in the culture medium. Conditions such as environmental factors, antibiotics, and inhibitors can induce H. Pylori to enter the VBNC state, but it is still not known whether H. pylori in the VBNC state can reactivate or not. Based on the cell membrane integrity and metabolic activity of H. pylori in the VBNC state, it can be detected by classical methods including direct microscopy of live bacteria and molecular biological methods such as reverse transcription-polymerase chain reaction. H. pylori in the VBNC state has been detected in water source and biological media. It has been also found that H. pylori can enter the VBNC state in artificially contaminated food, which poses challenges to public health and food safety. Therefore, it is of great significance to study the change pattern and detection methods of H. pylori in the VBNC state for the prevention and control of H. pylori in the VBNC state. It is valuable to further study the underlying mechanisms of H. pylori in the VBNC state.