Related Experiment Videos
The localization of a Mason-Pfizer monkey virus-related antigen in jaagsiekte tumour tissue and cell lines
The Onderstepoort Journal of Veterinary Research
|June 1, 1986
Abstract:
Mason-Pfizer monkey virus-related antigen was detected in 3 out of 5 jaagsiekte lungs examined using a direct immunoperoxidase staining technique with anti-MPMV p27 serum. Most of the antigen was localized in the alveolar lumina of the lesions. The reaction was further characterised on immune blots and found to involve a protein with a molecular mass of 29 000 daltons (JSRV p29). JSRV p29 antigen was also detected in 2 jaagsiekte cell lines.
Insights
Mason-Pfizer monkey virus (MPMV) antigen was found in sheep's jaagsiekte lungs and cell lines. This MPMV p27 antigen is linked to a 29,000-dalton protein (JSRV p29) in jaagsiekte lesions.
Area of Science:
- Veterinary Pathology
- Oncology
- Virology
Background:
- Jaagsiekte is a contagious retroviral lung cancer in sheep.
- The Mason-Pfizer monkey virus (MPMV) is a betaretrovirus.
- Detecting MPMV-related antigens can aid in diagnosing jaagsiekte.
Purpose of the Study:
- To detect MPMV-related antigen in jaagsiekte lungs.
- To characterize the specific protein involved.
- To investigate the presence of this antigen in jaagsiekte cell lines.
Main Methods:
- Direct immunoperoxidase staining using anti-MPMV p27 serum on sheep lung tissues.
- Immune blotting to characterize the antigen's molecular mass.
- Analysis of jaagsiekte cell lines for antigen presence.
Main Results:
- MPMV-related antigen was detected in 3 out of 5 examined jaagsiekte lungs.
- The antigen was primarily localized in the alveolar lumina of lesions.
- Immune blotting identified the antigen as a 29,000-dalton protein (JSRV p29).
- JSRV p29 antigen was also found in 2 jaagsiekte cell lines.
Conclusions:
- MPMV-related antigen, specifically JSRV p29, is present in jaagsiekte lesions and cell lines.
- Immunohistochemical detection of MPMV antigen can be a diagnostic tool for jaagsiekte.
- Further research into JSRV p29's role in jaagsiekte pathogenesis is warranted.