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Related Experiment Video

Updated: Oct 1, 2025

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
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Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

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[Application and progress in high-throughput sequencing technology for meat adulteration detection].

Yongqi Gan1, Manman Lu2, Qingniao Lai1

  • 1Guangxi Institute for Food and Drug Control, Nanning 530021, Guangxi, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|March 2, 2022
PubMed
Summary

DNA metabarcoding offers a rapid, precise method for detecting meat adulteration, safeguarding public health and religious practices. This technology simultaneously identifies multiple species in complex food samples, enhancing surveillance capabilities.

Keywords:
DNA barcodingfood safetyhigh-throughput sequencingmeat adulterationmetabarcoding technologyspecies identification

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Area of Science:

  • Food Science
  • Genetics
  • Analytical Chemistry

Background:

  • Meat adulteration poses significant public health and religious concerns.
  • Accurate detection of meat species is crucial for effective monitoring and control.
  • Traditional methods often lack the sensitivity and speed required for comprehensive surveillance.

Purpose of the Study:

  • To review the advancements in high-throughput sequencing (HTS) technology.
  • To provide an overview of DNA metabarcoding's technical characteristics and research methods.
  • To summarize the application, challenges, and future prospects of DNA metabarcoding in meat adulteration detection.

Main Methods:

  • Review of high-throughput sequencing technologies.
  • Analysis of DNA metabarcoding techniques for species identification.
  • Summary of recent research on DNA metabarcoding applications in meat products.

Main Results:

  • High-throughput sequencing has rapidly advanced over the past two decades.
  • DNA metabarcoding enables simultaneous, high-precision identification of multiple species in complex samples.
  • This technology demonstrates significant advantages for meat adulteration surveillance.

Conclusions:

  • DNA metabarcoding is a powerful tool for detecting meat adulteration.
  • Addressing current challenges will further enhance its application in food safety.
  • Future prospects indicate continued development and broader adoption of this technology.