Related Experiment Video
Updated: Aug 9, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
Published on: August 6, 2008
Fast quantification of nucleic acid hybrids by affinity-based hybrid collection
This study introduces a rapid hybridization technique for quantifying nucleic acids (DNA). The method uses a probe pair and affinity labeling for accurate detection, even with impure samples.
Area of Science:
- Molecular Biology
- Biochemistry
- Analytical Chemistry
Background:
- Accurate quantification of nucleic acids is crucial for molecular biology applications.
- Existing methods may be time-consuming or sensitive to sample impurities.
Purpose of the Study:
- To develop a novel, rapid, and quantitative hybridization technique for nucleic acid analysis.
- To enable direct analysis of biological samples without prior purification.
Main Methods:
- Utilized a probe pair for hybridization with target nucleic acid in solution.
- Employed affinity labeling (biotinylation) on one probe for hybrid capture using streptavidin-agarose.
- Quantified hybrids using radioiodine labeling on the second probe.
- Demonstrated non-isotopic detection via spectrophotometry after binding to microtitration wells.
Main Results:
- Achieved quantitative measurement of target DNA with a detection limit of 4 x 10^5 molecules (0.67 attomoles).
- The entire procedure was completed within 3 hours.
- The method demonstrated insensitivity to impurities in biological samples, allowing direct analysis.
Conclusions:
- The developed hybridization technique offers a fast, quantitative, and robust method for nucleic acid quantification.
- Its insensitivity to impurities and potential for non-isotopic detection make it suitable for various biological sample analyses.
More Related Videos
12:24DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
07:10Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
Published on: July 8, 2025
Related Concept Videos
Southern Blot
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
FISH - Fluorescent In-situ Hybridization
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
In-situ Hybridization
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...