Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.5K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual-mode pH-programmable enzymatic hydrogel system for on-demand glucose generation.

Soft matter·2026
Same author

Artificial allosteric protein switches with machine-learning-designed receptors.

Nature biotechnology·2026
Same author

Bioinspired silver nanoparticles from <i>Artemisia lerchiana</i> as durable electrodes for next-generation supercapacitors.

Physical chemistry chemical physics : PCCP·2026
Same author

High-Capacitance Gold Nanoparticles from <i>Rhus coriaria</i>: Green Synthesis, Characterization and Electrochemical Evaluation for Supercapacitor Technologies.

Micromachines·2026
Same author

Lanthanide-Controlled Protein Switches: Development and In Vitro and In Vivo Applications.

Angewandte Chemie (International ed. in English)·2025
Same author

pH-Driven Enzymatic Breakdown and Release of Catalase from Alginate Hydrogel.

ACS applied materials & interfaces·2024

Related Experiment Video

Updated: Oct 1, 2025

Bridging the Bio-Electronic Interface with Biofabrication
16:38

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

16.9K

Electrochemically produced local pH changes stimulating (bio)molecule release from pH-switchable

Ronaldo Badenhorst1, Vasantha Krishna Kadambar1, Madhura Bellare1

  • 1Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699-5810, USA. osmutok@clarkson.edu.

Physical Chemistry Chemical Physics : PCCP
|March 2, 2022
PubMed
Summary

Researchers developed a method to release biotinylated molecules using electrochemical pH changes. This technique utilizes controlled electrical potentials to trigger the release of molecular cargo from avidin complexes.

More Related Videos

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

9.1K
The Use of a &#946;-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

9.1K

Related Experiment Videos

Last Updated: Oct 1, 2025

Bridging the Bio-Electronic Interface with Biofabrication
16:38

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

16.9K
Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

9.1K
The Use of a &#946;-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

9.1K

Area of Science:

  • Electrochemistry
  • Biochemistry
  • Materials Science

Background:

  • Avidin-biotin interactions are crucial in biological systems and biochemical assays.
  • Controlled release of molecules is essential for various applications, including drug delivery and biosensing.
  • Electrochemical methods offer precise control over chemical reactions at electrode surfaces.

Purpose of the Study:

  • To demonstrate the electrochemical release of biotinylated molecules using pH-sensitive avidin complexes.
  • To investigate the use of electrochemical oxygen reduction and ascorbate oxidation for pH modulation.
  • To establish a system for triggered molecular release via electrical potentials.

Main Methods:

  • Immobilization of avidin-biotin complexes on electrode surfaces.
  • Electrochemical reactions (O2 reduction and ascorbate oxidation) to induce local pH changes.
  • Monitoring the dissociation of biotinylated molecules from avidin.

Main Results:

  • Successfully released biotinylated molecules by generating local pH changes near electrodes.
  • Nitro-avidin-biotin complex dissociation achieved via electrochemical O2 reduction (alkaline pH).
  • Avidin-iminobiotin complex dissociation achieved via ascorbate oxidation (acidic pH).
  • Release triggered by low electrical potentials (-0.4 V or 0.2 V).

Conclusions:

  • Electrochemical pH modulation provides a viable strategy for controlled release of biotinylated molecules.
  • This approach enables precise, electrically triggered cargo release from modified electrodes.
  • The developed systems show potential for applications in biosensing and targeted delivery.