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Updated: Aug 8, 2026
Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
Production of a monoclonal antibody specific for seminomas and dysgerminomas
Abstract:
A monoclonal antibody (M2A, IgG2a) was produced against a cultured human ovarian epithelial adenocarcinoma cell line, HEY. Monoclonal antibody M2A reacted with a glycoprotein of molecular weight 40,000 on the surface of HEY cells. The affinity constant of the monoclonal antibody M2A for HEY cells was 10(9) M-1, and the number of binding sites on HEY cells was 2 X 10(4) per cell. The monoclonal antibody produced positive immunoperoxidase staining of fetal (but not adult) testis and of seminomas and dysgerminomas but did not stain various normal adult tissues or other gonadal or extragonadal tumors. Monoclonal antibody M2A may be useful for confirming a histological diagnosis of seminoma and dysgerminoma.
Insights
Researchers developed a monoclonal antibody (M2A) that targets a specific glycoprotein on ovarian cancer cells. This antibody shows promise for diagnosing seminoma and dysgerminoma tumors.
Area of Science:
- Oncology
- Immunology
- Biochemistry
Background:
- Monoclonal antibodies are crucial tools in cancer research and diagnostics.
- Identifying specific tumor-associated antigens can improve diagnostic accuracy.
Purpose of the Study:
- To produce and characterize a novel monoclonal antibody (M2A) against human ovarian epithelial adenocarcinoma cells (HEY).
- To evaluate the diagnostic potential of M2A for specific tumor types.
Main Methods:
- Production of monoclonal antibody M2A against the HEY cell line.
- Characterization of M2A binding affinity and specificity using techniques like immunoperoxidase staining.
- Analysis of M2A reactivity with various human tissues and tumor types.
Main Results:
- Monoclonal antibody M2A specifically binds to a 40,000 molecular weight glycoprotein on HEY cells.
- M2A exhibits high affinity (10(9) M-1) and a defined number of binding sites (2 X 10(4) per cell).
- Positive staining was observed in fetal testis, seminomas, and dysgerminomas, but not in normal adult tissues or other tumors.
Conclusions:
- Monoclonal antibody M2A demonstrates specificity for seminomas and dysgerminomas.
- M2A has potential utility as a diagnostic marker for confirming histological diagnoses of these germ cell tumors.
