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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Published on: April 10, 2018
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Long noncoding RNA DANCR expression and its predictive value in patients with atherosclerosis
Fengxia An1, Yanliang Yin1, Weixian Ju1
1Department of Health, Dongying People's Hospital, Shandong, China.
Bioengineered
|March 2, 2022
Summary
The long noncoding RNA DANCR is upregulated in atherosclerosis patients and promotes disease progression by affecting vascular smooth muscle cell function. DANCR may serve as a diagnostic marker and therapeutic target for atherosclerosis.
Area of Science:
- Molecular Biology
- Cardiovascular Research
- Biochemistry
Background:
- Long noncoding RNAs (lncRNAs) are implicated in vascular diseases like atherosclerosis.
- The specific role of the lncRNA DANCR in atherosclerosis pathogenesis requires further investigation.
Purpose of the Study:
- To investigate DANCR expression levels in atherosclerosis patients.
- To determine if DANCR affects atherosclerosis progression and vascular smooth muscle cell (VSMC) behavior.
- To explore DANCR's potential as a diagnostic biomarker and therapeutic target.
Main Methods:
- Quantified DANCR expression in serum using RT-qPCR.
- Assessed diagnostic potential with ROC curve analysis.
- Evaluated effects on VSMC proliferation and migration using CCK-8 and Transwell assays.
- Investigated molecular interactions with miR-335-5p.
Main Results:
- DANCR expression was significantly higher in atherosclerosis patients compared to healthy controls.
- DANCR levels correlated positively with serum LDL-C, Hcy, and CRP.
- DANCR demonstrated high diagnostic accuracy for atherosclerosis detection.
- Knockdown of DANCR reduced VSMC proliferation and migration.
- DANCR was found to compete with miR-335-5p.
Conclusions:
- LncRNA DANCR is closely associated with atherosclerosis progression.
- DANCR may target miR-335-5p to influence VSMC function.
- DANCR presents a potential diagnostic biomarker and therapeutic target for atherosclerosis.
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