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Anticancer and Cytotoxicity Activity of Native and Modified Black Rice Flour on Colon Cancer Cell Lines

B Thanuja1, R Parimalavalli1, S Vijayanand2

  • 1Department of Food Science and Nutrition, Periyar University, Salem 636 011, Tamil Nadu, India.

Evidence-Based Complementary and Alternative Medicine : Ecam
|March 3, 2022
PubMed

Insights

Native black rice flour exhibits significant anticancer properties against colon cancer cells. Modified black rice flour showed weak cytotoxicity and was non-toxic to mouse embryo cells, indicating potential cancer-preventive phytochemicals.

Area of Science:

  • Food Science
  • Biotechnology
  • Cancer Research

Background:

  • Black rice flour contains phytochemicals with potential health benefits.
  • Understanding the cytotoxic effects of modified rice flour is crucial for food applications.

Purpose of the Study:

  • To evaluate the cytotoxicity of native and modified black rice flour.
  • To assess the anticancer potential against colon cancer (HCT116) and mouse embryo (3T3-L1) cell lines.

Main Methods:

  • Cytotoxicity was assessed using the MTT assay.
  • Rice flour samples underwent enzymatic modification and heat moisture treatment.
  • Half-maximal inhibitory concentration (IC50) values were determined for both cell lines.

Main Results:

  • Native black rice flour demonstrated significant cytotoxic and anticancer potential against HCT116 cells (IC50: 255.78 µg/mL).
  • Modified black rice flour showed weak cytotoxicity against HCT116 cells (IC50: 340.85 µg/mL).
  • Native and modified black rice flour exhibited weak to non-toxic effects on 3T3-L1 cells (IC50: 345.96 µg/mL and 1106.94 µg/mL, respectively).

Conclusions:

  • Native black rice flour possesses significant anticancer potential against colon cancer.
  • Modified black rice flour is non-toxic and may be suitable for food applications.
  • Phytochemicals in black rice flour may play a role in colon cancer prevention.

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