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Updated: Oct 1, 2025

Isolation and Excision of Murine Aorta; A Versatile Technique in the Study of Cardiovascular Disease

Isolation and Excision of Murine Aorta; A Versatile Technique in the Study of Cardiovascular Disease

Published on: November 24, 2014

Isolation of Mouse Aortic RNA for Transcriptomics.

Rosa M Nevado^1,2, Magda R Hamczyk^2,3, Vicente Andrés^4,5

¹Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain.

Methods in Molecular Biology (Clifton, N.J.)

|March 3, 2022

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Aging alters the arterial wall, increasing vascular disease risk. This study details RNA extraction methods from mouse aorta layers to analyze gene expression and understand disease origins for new treatments.

Area of Science:

Vascular Biology
Transcriptomics
Aging Research

Background:

Aging is linked to arterial wall changes, driving vascular diseases like heart attack and stroke.
The arterial wall's three layers (intima, media, adventitia) have unique roles in disease development.
Understanding layer-specific transcriptomic changes is crucial for novel therapeutic strategies.

Purpose of the Study:

To present protocols for RNA extraction from distinct layers of the mouse aorta.
To enable detailed transcriptomic analysis of the aging arterial wall.
To facilitate research into the etiopathology of vascular diseases.

Main Methods:

Detailed protocols for total RNA extraction from the entire mouse aorta.
Specific methods for isolating RNA from intima, media, and adventitia layers.

Keywords:

Adventitia Aorta Intima Media RNA extraction Transcriptomics Vascular disease

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Preparation of samples for subsequent transcriptomic analysis.

Main Results:

Established reliable RNA extraction methods for whole aorta and individual layers.
Enabled layer-specific transcriptomic profiling of the aging arterial wall.
Provided a foundation for investigating age-related vascular gene expression patterns.

Conclusions:

Accurate RNA extraction from aortic layers is feasible.
This methodology supports in-depth transcriptomic studies of vascular aging.
The findings contribute to understanding vascular disease mechanisms and developing targeted therapies.