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Related Experiment Video

Updated: Oct 1, 2025

Intravital Microscopy of Monocyte Homing and Tumor-Related Angiogenesis in a Murine Model of Peripheral Arterial Disease
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Intravital Microscopy in Atherosclerosis Research.

Georg Wissmeyer1, Mohamad B Kassab1, Yoichiro Kawamura1

  • 1Cardiovascular Research Center, Division of Cardiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 3, 2022
PubMed
Summary

Multiphoton microscopy offers advanced in vivo imaging for studying atherosclerosis, a lipid-driven inflammatory arterial disease. This technique provides subcellular resolution to understand disease mechanisms and complications.

Keywords:
AtherosclerosisGated microscopyInflammationIntravital imagingIntravital microscopyMolecular imagingMultiphoton microscopy

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Area of Science:

  • Cardiovascular Biology
  • Biomedical Imaging
  • Translational Medicine

Background:

  • Atherosclerosis is a major cause of cardiovascular disease, characterized by arterial narrowing due to lipid accumulation and inflammation.
  • Understanding the cellular mechanisms of atherosclerosis is crucial for developing effective treatments.
  • Intravital microscopy has advanced in vivo studies of disease processes.

Purpose of the Study:

  • To describe the application of state-of-the-art multiphoton microscopy in studying murine atherosclerosis.
  • To highlight the capabilities of multiphoton microscopy for in vivo imaging of atherosclerosis.

Main Methods:

  • Utilizing multiphoton microscopy, a technique employing two-photon-excited fluorescence and second-harmonic generation.
  • Achieving subcellular resolution and enhanced imaging depths compared to traditional microscopy methods.
  • Applying these advanced imaging techniques to investigate the biological processes in murine models of atherosclerosis.

Main Results:

  • Multiphoton microscopy enables detailed, in vivo visualization of cellular processes in atherosclerosis.
  • The technique provides superior resolution and depth for studying disease progression.
  • This approach facilitates a deeper mechanistic understanding of atherosclerosis onset and development.

Conclusions:

  • Multiphoton microscopy is a powerful tool for advancing atherosclerosis research.
  • The described methods offer new avenues for in vivo investigation of arterial diseases.
  • This imaging modality holds significant promise for future discoveries in cardiovascular research.