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Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography
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Permanent preservation of whole alizarin red S skeletons by clearing and embedding in polyester resins

D Chappard, C Alexandre, S Palle

    Stain Technology
    |May 1, 1986
    PubMed

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    Fixation and Sectioning01:03

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    Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
    The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...