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Updated: Oct 1, 2025
05:23
Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance
Published on: March 7, 2025
512
Changes in Gastric Mucosal Glycosylation Before and After Helicobacter pylori Eradication Using Lectin Microarray
Ryo Ogawa1, Tadayoshi Okimoto1, Masaaki Kodama2
1Department of Gastroenterology, Faculty of Medicine, Oita University, Oita, Japan.
Summary
Helicobacter pylori infection alters gastric mucosal glycosylation patterns, affecting cell functions. These changes are reversible upon successful H. pylori eradication, suggesting a dynamic relationship.
Area of Science:
- Glycobiology
- Gastroenterology
- Microbiology
Background:
- Altered glycosylation is linked to cell proliferation, differentiation, adhesion, and carcinogenesis.
- Helicobacter pylori infection is a significant factor in gastric diseases.
Purpose of the Study:
- To investigate the impact of Helicobacter pylori infection on gastric mucosal glycosylation.
- To assess the reversibility of glycosylation changes after H. pylori eradication.
Main Methods:
- Utilized a lectin microarray system to analyze glycosylation patterns.
- Employed laser capture microdissection to isolate gastric gland cells from biopsy samples.
- Compared glycosylation profiles in H. pylori-infected, non-infected, and eradicated patients.
Main Results:
- H. pylori infection significantly altered lectin-glycan interactions in the gastric mucosa.
- Specific lectin binding increased or decreased upon infection and reversed after eradication.
- Changes in jacalin, MPA, Lycopersicon esculentum lectin, and Sambucus lectins were noted in specific gastric regions.
Conclusions:
- Helicobacter pylori infection induces reversible changes in gastric mucosal glycosylation.
- These glycosylation alterations are associated with various cancers.
- H. pylori eradication can restore normal glycosylation patterns.
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