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Research perspectives-Pipelines to human tendon transcriptomics.

Lorenzo Ramos-Mucci1, Paula Sarmiento2, Dianne Little2,3

  • 1Nuffield Department of Orthopaedics Rheumatology and Musculoskeletal Science, University of Oxford, Oxford, UK.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|March 3, 2022
PubMed
Summary

Human tendon transcriptomics research faces challenges due to tissue availability and quality. This review highlights donor factors, tissue characteristics, and extraction methods impacting human tendon transcriptome studies.

Keywords:
publication guidelinesquality controlsequencingtendontissue source

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Area of Science:

  • Musculoskeletal biology
  • Transcriptomics
  • Genomics

Background:

  • Tendon transcriptomics studies aim to understand human tendon pathology by characterizing microenvironments.
  • The transcriptome integrates genomic, proteomic, and metabolomic data within the tendon cellular niche.
  • Challenges include limited availability and variable quality of human tendon tissue, often necessitating animal models.

Purpose of the Study:

  • To review variability in human donor and procurement factors affecting tendon transcriptomes.
  • To discuss tissue-specific challenges in cell, nuclei, and RNA extraction for tendon research.
  • To propose guidelines for future human tendon transcriptomics studies.

Main Methods:

  • Review of existing literature on human tendon transcriptomics.
  • Analysis of factors influencing human tendon tissue quality and transcriptome relevance.
  • Discussion of extraction, library preparation, and quality control methods.

Main Results:

  • Human donor factors (cadaveric vs. clinical remnant), age, sex, and health impact tendon transcriptomes.
  • Tendon tissue presents challenges: dense matrix, low cellularity, variable RNA yield and quality.
  • These factors are critical for single-cell and single-nuclei transcriptomics.

Conclusions:

  • Standardized guidelines for human tendon procurement, processing, and reporting are needed.
  • Attention to methodological details is crucial for reliable and translational tendon research.
  • Addressing these challenges will advance understanding of human tendon pathology.