Related Experiment Video
Updated: Oct 1, 2025

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
Published on: June 19, 2015
Abundance of integrons in halophilic bacteria
Sarah Sonbol1, Rania Siam1,2
1Biology Department and the Graduate Program of Biotechnology, School of Sciences and Engineering, the American University in Cairo, New Cairo 11835, Cairo, Egypt.
Abstract:
Integrons are genetic platforms used for expressing open reading frames (ORFs) arranged in gene cassettes. Excision and integration of gene cassettes are controlled by their associated integron integrase (IntI). Using IntegronFinder software, we analyzed all complete halophilic genomes available in the HaloDom database, along with selected partial halophilic genomes. We identified 18 new complete bacterial integrons and 46 clusters of attC sites lacking a neighboring integron integrase (CALINs). Different classes of insertion sequences (ISs) were also identified within and near integrons and CALINs, with an abundance of IS1182 elements and different ISs that can presumably mobilize adjacent genomic structures. Different promoters of intI genes (PintI) showed nearby binding sites for arginine repressor (ArgR), raising the possibility that IntI expression and recombination activity are regulated by these proteins. Our findings revealed the existence of new integrons in halophilic bacteria with possible adaptive roles.
Related Concept Videos
Diversity of Archaea IV
Hyperthermophilic Bacteria
Diversity of Archaea I
Bacterial Phylum Proteobacteria
Plasmids
Anoxygenic Phototrophic Bacteria

